J. Shawn Goodwin, Ph.D.
Director of Fluorescence Microscopy Core and Assistant Professor
Department of Cancer Biology,
Department of Biomedical Sciences

(615) 327-6686
WBS – 3109
1005 Dr. D.B. Todd Boulevard
Nashville, TN  37208

jgoodwin@mmc.edu

Lab URL

CV or Bio (PDF)

B.S., Chemistry, Millsaps College
Ph.D., Chemistry and Biochemistry, University of Southern Mississippi
Postdoctoral, Department of Molecular Physiology and Biophysics in the laboratory of Anne Kenworthy, Vanderbilt University

Cellular biophysics, quantitative fluorescence microscopy, and all aspects of light microscopy.

Dr. Goodwin’s primary role as Director of the Morphology Core is to establish and maintain a fluorescence microscopy resource for the faculty and their trainees and to integrate the resources of fluorescence and bright-field microscopy with histology and immunohistochemistry.  The core offers consultation on instrumentation and experimental design, training on instrumentation, and collaboration opportunities.  Please see the Core website for more information.

Dr. Goodwin’s own research focuses on aspects of protein trafficking, including how altered levels of cholesterol, which influence lipid rafts, affect the trafficking and membrane diffusion of both raft and non-raft proteins.  These studies include fluorescence recovery after photobleaching (FRAP), and fluorescence loss in photobleaching.  By collaborations with other investigations, he is examining both trafficking and signaling, including realtime detection of changes in intracellular Ca2+ levels, detected with Ca2+ - sensitive fluorophores.

Goodwin, J. Shawn, Kimberly R. Drake, Carl Rogers, Vi K. Chiu, Jennifer Lippincott-Schwartz, Mark R. Philips, Anne K. Kenworthy (2005) Depalmitoylated Ras traffics to and from the Golgi complex via a non-vesicular pathway, Journal of Cell Biology, 170, 261-272.

Goodwin, J. Shawn, Kimberly R. Drake, Catha L. Remmert, Anne K. Kenworthy (2005) Ras diffusion is sensitive to plasma membrane viscosity, Biophysical Journal, 89, 1398-1410.

Goodwin, J. Shawn and Anne K. Kenworthy (2005) Photobleaching approaches to investigate diffusional mobility and trafficking of Ras in living cells, Methods, 37, 154-164.