Director of Fluorescence Microscopy Core and Assistant Professor
Department of Cancer Biology
Department of Biomedical Sciences
West Basic Science Bldg. Room 3109

Phone: (615) 327-6686
jgoodwin@mmc.edu

Cellular biophysics, quantitative fluorescence microscopy, and all aspects of light microscopy.

Dr. Goodwin's primary role as Director of the Morphology Core is to establish and maintain a fluorescence microscopy resource for the faculty and their trainees and to integrate the resources of the fluorescence and bright-field microscopy with histology and immunohistochemistry. The core offers consultation on instrumentation and experimental design, training on instumentation, and collaboration opportunities. Please see the Core website for more information.

Dr. Goodwin's own research focuses on aspects of protein trafficking, including how altered levels of cholesterol, which influence lipid rafts, affect the trafficking and membrane diffusion of both raft and non-raft proteins. These studies include fluorescence recovery after photobleaching (FRAP), and fluorescence loss in photobleaching. By collaborations with other investigations, he is examining both trafficking and signaling, including realtime detection of changes in intracellular Ca2+ levels, detected with Ca2+ - sensitive fluorophores.

1. Mines, Marjelo A., J. Shawn Goodwin, Lee E. Limbird, Fei-Fei Cui, Guo-Huang Fan (2009) Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK activation, J Biol Chem. Feb 27;284(9):5742-52.


2. Goodwin, J. Shawn, Namita Sen, Jonathan A. Javitch, Louis J. DeFelice, Habibeh Khoshbouei (2009) In vitro and in vivo studies reveal differential effects of methamphetamine and amphetamine on dopamine transporter functions, J Biol Chem. Jan 30;284(5):2978-89.


3. Ding, Yun, Aimin Qiao, Ziqing Wang, J. Shawn Goodwin, Eun-Sook Lee, Michelle L. Block, Matthew Allsbrook, Michael McDonald, and Guo-Huang Fan (2008) Retinoic Acid Attenuates beta-Amyloid Deposition and Rescues Memory Deficits in an Alzheimer Disease Transgenic Mouse Model, J Neurosci, Nov 5;28(45):11622-34.


4. DongFang Wu, Hong Yang, YanFeng Zhao, Chakradhari Sharan, J. Shawn Goodwin, Lichun Zhou, Yang Guo and ZhongMao Guo (2008) 2-Aminopurine inhibits apolipoprotein E-deficient lipoprotein-induced foam cell formation, J Pharmacol Exp Ther, 326(2): 395-405.


5. Ding,Yun, Li Zhang, J. Shawn Goodwin, Ziqing Wang, Bingdong Liu, Jingwu Zhang, Guo-Huang Fan (2008) Plectin regulates the signaling and trafficking of the HIV-1 co-receptor CXCR4 and plays a role in HIV-1 infection, Exp Cell Res., 314: 590-602.

For more of Dr. Goodwin's publications, see his Biosketch