Shandon Cadenza automated multi-slide immunostainer
This is a fully programmable automated immunostainer, designed to perform all stages of immunostaining (ICC) from the application of blocking agents up to and including counterstaining.
- It can accommodate up to 9 different primary antisera.
- It is capable of processing 20 slides at one time.
Nikon TE2000-U C1 confocal laser scanning microscope
This confocal has 488 nm, 543 nm, and 633 nm laser lines to excite fluorophores with compatible excitation characteristics. Fluorescence emission is collected with 505-550 nm, 560-615 nm, and 650 nm filter sets, respectively, in independent channels. The microscope is also equipped with a fourth detector to collect light for differential interference contrast (DIC) imaging.
- There are 10x, 20x, 40x, 60x, and 100x objective lenses available for use on this
microscope. They are all capable of imaging with DIC, making it possible to do bright-field
- Motorized Z-plane movement allows for optical sectioning through a sample. The EZ C1 confocal software can be used subsequently to reconstruct serial Z-plane images into a 3D rendering.
Images obtained with this microscope:
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Nikon TE2000-E wide-field fluorescence microscope
The Nikon TE2000-E is a fully automated wide-field fluorescence microscope that is equipped for diverse imaging.
- A motorized Z-focusing motor enables collection of in-focus images in different image
planes. This utility is most often used in acquiring images for deconvolution and
- It has a xenon lamp for fluorescence excitation, which is an excellent light source
for calcium imaging with Fura-2 and UV-Vis fluorophores.
- A very sensitive CoolSNAP HQ2 camera is ideal for fluorescence imaging, as it produces
very little noise and allows for faster integration times.
- A 5 mega pixel color camera is available for non-fluorescent samples and is ideal
for histological stains.
- There are 4x and 10x phase lenses available as well as high NA 20x, 40x, 60x, and
100x DIC lenses.
- A computer controlled excitation filter wheel allows for fast, automated collection
- Filter sets are available for UV, blue, green, and red excitation and emitted light
is collected with the corresponding band pass or long pass emission filters.
- Filter sets are available for calcium ratio imaging, specifically Fura-2 AM.
- A motorized stage in conjuction with Nikon NIS Elements or Stereologer software allows
for the collection of images in multiple, specified locations.
- Nikon NIS Elements Advanced Research imaging software is capable of 6 dimensions (6D) of acquisition. Up to six combinations of wavelength, time, x-, y-, and/or z-positions are possible to control with this software.
These are images are of Trypanosoma brucei. They were acquired with a Nikon TE2000U C1 laser scanning microscope equipped a 100x 1.4 NA DIC objective. The sample was excited with a 488nm laser, and the emitted light was collected using 505-550nm band pass. The image of the T. brucei on the left was acquired using DIC and on the right by imaging MitoTracker Green.
Rat embryo fibroblasts were labeled with Hoechst (blue, nucleus), Alexa Fluor 488-phalloidin (green, actin), and MitoTracker Red (orange, mitochondria). The image was acquired using a Nikon TE2000E equipped with a 20x 0.75 NA objective lens and a CoolSNAP HQ2 camera.
Picture of Nikon TE2000-U C1
Picture of Nikon TE2000-E